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According to the provided data, the Estudiantes Rio Cuarto team showed a strong performance in their last match, winning with a score of 1-0. They also won both previous encounters against Defensores Unidos without conceding a goal. This could indicate an advantage and confidence for Estudiantes. They have a higher position in the league table compared to Defensores Unidos, finishing 4th in the previous season. At the same time, Defensores Unidos suffered a defeat in their last match of the first round. This could negatively impact their morale and confidence on the field. However, keep in mind that football always has room for surprises and teams can vary greatly from game to game. It will be interesting to see how these two teams will play against each other in this match.